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isre ifnb1 promoter plasmid  (Addgene inc)


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    Structured Review

    Addgene inc isre ifnb1 promoter plasmid
    Isre Ifnb1 Promoter Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 63 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/isre+ifnb1+promoter+plasmid/IFN-Beta_pGL3+(Plasmid+%23102597)/10__70962_slash_jhi__20250009-184-14-20
    Average 94 stars, based on 63 article reviews
    isre ifnb1 promoter plasmid - by Bioz Stars, 2026-09
    94/100 stars

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    Transfection:

    Article Title: IRF4 haploinsufficiency in a multiplex family with Whipple’s disease
    Article Snippet: Antibody binding was detected with the Clarity Western ECL Substrate, (#1705060; Bio-Rad) and SuperSignal West Pico PLUS Chemiluminescent Substrate Kits (#34580; Thermo Fisher Scientific). .. HEK293T cells were plated in 96-well plates (25,000 cell/well) and transiently transfected with the ISRE-IFNB1 promoter plasmid (pGL3 backbone, #102597; Addgene) (100 ng/well and 100 μl DMEM-10% FBS medium), the pRL-SV40 vector (#E2231; Promega, 40 ng/well), and IRF4 WT or IRF4 mutant pcDNA3.1D V5-His-TOPO IRF4 plasmid (0.5 ng/well), with X-tremeGENE 9 DNA Transfection Reagent kit (#6365809001; Roche), according to the manufacturer’s instructions. .. Cells were lysed, 24 h later, with Passive Lysis 5X Buffer (diluted to 1X, # E1910; Promega).

    Plasmid Preparation:

    Article Title: IRF4 haploinsufficiency in a multiplex family with Whipple’s disease
    Article Snippet: Antibody binding was detected with the Clarity Western ECL Substrate, (#1705060; Bio-Rad) and SuperSignal West Pico PLUS Chemiluminescent Substrate Kits (#34580; Thermo Fisher Scientific). .. HEK293T cells were plated in 96-well plates (25,000 cell/well) and transiently transfected with the ISRE-IFNB1 promoter plasmid (pGL3 backbone, #102597; Addgene) (100 ng/well and 100 μl DMEM-10% FBS medium), the pRL-SV40 vector (#E2231; Promega, 40 ng/well), and IRF4 WT or IRF4 mutant pcDNA3.1D V5-His-TOPO IRF4 plasmid (0.5 ng/well), with X-tremeGENE 9 DNA Transfection Reagent kit (#6365809001; Roche), according to the manufacturer’s instructions. .. Cells were lysed, 24 h later, with Passive Lysis 5X Buffer (diluted to 1X, # E1910; Promega).

    Mutagenesis:

    Article Title: IRF4 haploinsufficiency in a multiplex family with Whipple’s disease
    Article Snippet: Antibody binding was detected with the Clarity Western ECL Substrate, (#1705060; Bio-Rad) and SuperSignal West Pico PLUS Chemiluminescent Substrate Kits (#34580; Thermo Fisher Scientific). .. HEK293T cells were plated in 96-well plates (25,000 cell/well) and transiently transfected with the ISRE-IFNB1 promoter plasmid (pGL3 backbone, #102597; Addgene) (100 ng/well and 100 μl DMEM-10% FBS medium), the pRL-SV40 vector (#E2231; Promega, 40 ng/well), and IRF4 WT or IRF4 mutant pcDNA3.1D V5-His-TOPO IRF4 plasmid (0.5 ng/well), with X-tremeGENE 9 DNA Transfection Reagent kit (#6365809001; Roche), according to the manufacturer’s instructions. .. Cells were lysed, 24 h later, with Passive Lysis 5X Buffer (diluted to 1X, # E1910; Promega).



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    isre ifnb1 promoter plasmid  (Addgene inc)
    94
    Addgene inc isre ifnb1 promoter plasmid
    Isre Ifnb1 Promoter Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/isre+ifnb1+promoter+plasmid/IFN-Beta_pGL3+(Plasmid+%23102597)/10__70962_slash_jhi__20250009-184-14-20
    Average 94 stars, based on 1 article reviews
    isre ifnb1 promoter plasmid - by Bioz Stars, 2026-09
    94/100 stars
    		  Buy from Supplier

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